Thank you for your question. As far as the accuracy of the test, it has a greater than 99% correlation with ELISA. All 252 leaf samples and 248 root samples that were used in the validation were pre-screened with ELISA and run in duplicate with the HVX test strip. There were two samples (one duplicate sample) that did not initially match with the Elisa results but when retested with another strip it did confirm. It is believed that the reason for this was user error on the first attempt. In regards to testing plants with no symptoms, Hosta virus X is a Potexvirus. That fact being said, it is bad for the plant but good for the test. This Potexvirus, HVX tends to be distributed throughout the infected plant and tends to reach fairly high levels as well. It is pretty well known that an HVX infection can remain asymptomatic years but as long as the virus is present, the strip should have no problem picking it up.
- Steve Lutes